Regulation of β-Disaccharide Accumulation by β-Glucosidase Inhibitors to Enhance Cellulase Production in Trichoderma reesei

Trichoderma reesei is a high-yield producer of cellulase for applications in lignocellulosic biomass conversion, but its production requires induction. A mixture glucose and β-disaccharide has been demonstrated to achieve high-level production. However, as inducers, β-disaccharides are prone be hydrolyzed by β-glucosidase (BGL) during fermentation, therefore need supplemented through feeding overcome this problem. Here, miglitol, an α-glucosidase inhibitor, was investigated BGL exhibited IC50 value 2.93 μg/mL. The titer more than two-fold when miglitol added the fermentation medium T. reesei. This method similar prokaryotic expression system using unmetabolized isopropyl-β-D-thiogalactopyranoside (IPTG) inducer instead lactose continuously induce gene expression. activity not enhanced with inhibition or cellulose used inducer, which that transglycosidase important inducible cellulose. novel demonstrates potential stimulating provides promising protein